cervical cancer derived cell lines Search Results


hela human cervical cancer  (National Centre for Cell Science)
90
National Centre for Cell Science hela human cervical cancer
A. Representative images of <t>HeLa</t> <t>and</t> <t>HEK293T</t> cells showing localization of BG4, the G4 binding antibody to mitochondria following immunofluorescence study. Nucleus is stained with DAPI (blue color), mitochondria with MitoTracker DR (red) and BG4 with Alexa-Fluor 488 (green). A merged image is shown with merge of red and green as depicted by Coste’s mask (colocalization is represented as a white dot). B, C. Quantitation showing colocalization of BG4 with MitoTracker indicated as dot plots. The colocalization was quantified using Mander’s colocalization coefficient (ImageJ software) analyzing a minimum of 100 cells as red over green (B) and green over red (C). D. Representative image of rho(0) cell showing localization of BG4 to mitochondria following immunofluorescence as investigated in panel A. E. Quantitation showing comparison of colocalization of BG4 between HeLa cells and rho(0) cells is shown as dot plots. The colocalization was quantified using Mander’s colocalization coefficient analyzing a minimum of 50 cells as red over green. F. BG4 bound mtDNA was purified after reverse crosslinking and used for real-time PCR using primers derived from different regions of the mitochondrial genome, which include 5 G-quadruplex forming regions and 10 random regions. Input DNA served as template control. No antibody control was also used. Bars in blue (first 5) are for G-quadruplex forming regions, while in green (last 10) are for random regions. Y-axis depicts threshold Ct value obtained following real time PCR for each primer. Error bar represents mean ± SEM. G. Agarose gel profile showing the amplification of Input DNA (left panel) and BG4 pull down DNA (right panel). “M” denotes 100 bp ladder.
Hela Human Cervical Cancer, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hela human cervical cancer/product/National Centre for Cell Science
Average 90 stars, based on 1 article reviews
hela human cervical cancer - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
cervical cancer cell lines siha  (National Centre for Cell Science)
90
National Centre for Cell Science cervical cancer cell lines siha
A. Representative images of <t>HeLa</t> <t>and</t> <t>HEK293T</t> cells showing localization of BG4, the G4 binding antibody to mitochondria following immunofluorescence study. Nucleus is stained with DAPI (blue color), mitochondria with MitoTracker DR (red) and BG4 with Alexa-Fluor 488 (green). A merged image is shown with merge of red and green as depicted by Coste’s mask (colocalization is represented as a white dot). B, C. Quantitation showing colocalization of BG4 with MitoTracker indicated as dot plots. The colocalization was quantified using Mander’s colocalization coefficient (ImageJ software) analyzing a minimum of 100 cells as red over green (B) and green over red (C). D. Representative image of rho(0) cell showing localization of BG4 to mitochondria following immunofluorescence as investigated in panel A. E. Quantitation showing comparison of colocalization of BG4 between HeLa cells and rho(0) cells is shown as dot plots. The colocalization was quantified using Mander’s colocalization coefficient analyzing a minimum of 50 cells as red over green. F. BG4 bound mtDNA was purified after reverse crosslinking and used for real-time PCR using primers derived from different regions of the mitochondrial genome, which include 5 G-quadruplex forming regions and 10 random regions. Input DNA served as template control. No antibody control was also used. Bars in blue (first 5) are for G-quadruplex forming regions, while in green (last 10) are for random regions. Y-axis depicts threshold Ct value obtained following real time PCR for each primer. Error bar represents mean ± SEM. G. Agarose gel profile showing the amplification of Input DNA (left panel) and BG4 pull down DNA (right panel). “M” denotes 100 bp ladder.
Cervical Cancer Cell Lines Siha, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cervical cancer cell lines siha/product/National Centre for Cell Science
Average 90 stars, based on 1 article reviews
cervical cancer cell lines siha - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
cervical cancer cells  (Korean Cell Line Bank)
90
Korean Cell Line Bank cervical cancer cells
A. Representative images of <t>HeLa</t> <t>and</t> <t>HEK293T</t> cells showing localization of BG4, the G4 binding antibody to mitochondria following immunofluorescence study. Nucleus is stained with DAPI (blue color), mitochondria with MitoTracker DR (red) and BG4 with Alexa-Fluor 488 (green). A merged image is shown with merge of red and green as depicted by Coste’s mask (colocalization is represented as a white dot). B, C. Quantitation showing colocalization of BG4 with MitoTracker indicated as dot plots. The colocalization was quantified using Mander’s colocalization coefficient (ImageJ software) analyzing a minimum of 100 cells as red over green (B) and green over red (C). D. Representative image of rho(0) cell showing localization of BG4 to mitochondria following immunofluorescence as investigated in panel A. E. Quantitation showing comparison of colocalization of BG4 between HeLa cells and rho(0) cells is shown as dot plots. The colocalization was quantified using Mander’s colocalization coefficient analyzing a minimum of 50 cells as red over green. F. BG4 bound mtDNA was purified after reverse crosslinking and used for real-time PCR using primers derived from different regions of the mitochondrial genome, which include 5 G-quadruplex forming regions and 10 random regions. Input DNA served as template control. No antibody control was also used. Bars in blue (first 5) are for G-quadruplex forming regions, while in green (last 10) are for random regions. Y-axis depicts threshold Ct value obtained following real time PCR for each primer. Error bar represents mean ± SEM. G. Agarose gel profile showing the amplification of Input DNA (left panel) and BG4 pull down DNA (right panel). “M” denotes 100 bp ladder.
Cervical Cancer Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cervical cancer cells/product/Korean Cell Line Bank
Average 90 stars, based on 1 article reviews
cervical cancer cells - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
human cervical cancer hela cell line  (BioVector NTCC)
90
BioVector NTCC human cervical cancer hela cell line
A. Representative images of <t>HeLa</t> <t>and</t> <t>HEK293T</t> cells showing localization of BG4, the G4 binding antibody to mitochondria following immunofluorescence study. Nucleus is stained with DAPI (blue color), mitochondria with MitoTracker DR (red) and BG4 with Alexa-Fluor 488 (green). A merged image is shown with merge of red and green as depicted by Coste’s mask (colocalization is represented as a white dot). B, C. Quantitation showing colocalization of BG4 with MitoTracker indicated as dot plots. The colocalization was quantified using Mander’s colocalization coefficient (ImageJ software) analyzing a minimum of 100 cells as red over green (B) and green over red (C). D. Representative image of rho(0) cell showing localization of BG4 to mitochondria following immunofluorescence as investigated in panel A. E. Quantitation showing comparison of colocalization of BG4 between HeLa cells and rho(0) cells is shown as dot plots. The colocalization was quantified using Mander’s colocalization coefficient analyzing a minimum of 50 cells as red over green. F. BG4 bound mtDNA was purified after reverse crosslinking and used for real-time PCR using primers derived from different regions of the mitochondrial genome, which include 5 G-quadruplex forming regions and 10 random regions. Input DNA served as template control. No antibody control was also used. Bars in blue (first 5) are for G-quadruplex forming regions, while in green (last 10) are for random regions. Y-axis depicts threshold Ct value obtained following real time PCR for each primer. Error bar represents mean ± SEM. G. Agarose gel profile showing the amplification of Input DNA (left panel) and BG4 pull down DNA (right panel). “M” denotes 100 bp ladder.
Human Cervical Cancer Hela Cell Line, supplied by BioVector NTCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cervical cancer hela cell line/product/BioVector NTCC
Average 90 stars, based on 1 article reviews
human cervical cancer hela cell line - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
human cervical cancer cell line hela  (DS Pharma Biomedical)
90
DS Pharma Biomedical human cervical cancer cell line hela
A. Representative images of <t>HeLa</t> <t>and</t> <t>HEK293T</t> cells showing localization of BG4, the G4 binding antibody to mitochondria following immunofluorescence study. Nucleus is stained with DAPI (blue color), mitochondria with MitoTracker DR (red) and BG4 with Alexa-Fluor 488 (green). A merged image is shown with merge of red and green as depicted by Coste’s mask (colocalization is represented as a white dot). B, C. Quantitation showing colocalization of BG4 with MitoTracker indicated as dot plots. The colocalization was quantified using Mander’s colocalization coefficient (ImageJ software) analyzing a minimum of 100 cells as red over green (B) and green over red (C). D. Representative image of rho(0) cell showing localization of BG4 to mitochondria following immunofluorescence as investigated in panel A. E. Quantitation showing comparison of colocalization of BG4 between HeLa cells and rho(0) cells is shown as dot plots. The colocalization was quantified using Mander’s colocalization coefficient analyzing a minimum of 50 cells as red over green. F. BG4 bound mtDNA was purified after reverse crosslinking and used for real-time PCR using primers derived from different regions of the mitochondrial genome, which include 5 G-quadruplex forming regions and 10 random regions. Input DNA served as template control. No antibody control was also used. Bars in blue (first 5) are for G-quadruplex forming regions, while in green (last 10) are for random regions. Y-axis depicts threshold Ct value obtained following real time PCR for each primer. Error bar represents mean ± SEM. G. Agarose gel profile showing the amplification of Input DNA (left panel) and BG4 pull down DNA (right panel). “M” denotes 100 bp ladder.
Human Cervical Cancer Cell Line Hela, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cervical cancer cell line hela/product/DS Pharma Biomedical
Average 90 stars, based on 1 article reviews
human cervical cancer cell line hela - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
human cc cell lines siha  (Procell Inc)
90
Procell Inc human cc cell lines siha
A. Representative images of <t>HeLa</t> <t>and</t> <t>HEK293T</t> cells showing localization of BG4, the G4 binding antibody to mitochondria following immunofluorescence study. Nucleus is stained with DAPI (blue color), mitochondria with MitoTracker DR (red) and BG4 with Alexa-Fluor 488 (green). A merged image is shown with merge of red and green as depicted by Coste’s mask (colocalization is represented as a white dot). B, C. Quantitation showing colocalization of BG4 with MitoTracker indicated as dot plots. The colocalization was quantified using Mander’s colocalization coefficient (ImageJ software) analyzing a minimum of 100 cells as red over green (B) and green over red (C). D. Representative image of rho(0) cell showing localization of BG4 to mitochondria following immunofluorescence as investigated in panel A. E. Quantitation showing comparison of colocalization of BG4 between HeLa cells and rho(0) cells is shown as dot plots. The colocalization was quantified using Mander’s colocalization coefficient analyzing a minimum of 50 cells as red over green. F. BG4 bound mtDNA was purified after reverse crosslinking and used for real-time PCR using primers derived from different regions of the mitochondrial genome, which include 5 G-quadruplex forming regions and 10 random regions. Input DNA served as template control. No antibody control was also used. Bars in blue (first 5) are for G-quadruplex forming regions, while in green (last 10) are for random regions. Y-axis depicts threshold Ct value obtained following real time PCR for each primer. Error bar represents mean ± SEM. G. Agarose gel profile showing the amplification of Input DNA (left panel) and BG4 pull down DNA (right panel). “M” denotes 100 bp ladder.
Human Cc Cell Lines Siha, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cc cell lines siha/product/Procell Inc
Average 90 stars, based on 1 article reviews
human cc cell lines siha - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
human epithelioid cervical adenocarcinoma (hela) cells  (Korean Cell Line Bank)
90
Korean Cell Line Bank human epithelioid cervical adenocarcinoma (hela) cells
E2F8 expression in cervical cancer cell lines and correlation with suppressed cell proliferation. (A, B) The expression levels of E2F8 RNA and protein were significantly higher in some cervical cancer cell lines (CaSki, <t>HeLa,</t> and ME180) compared to that in normal control cells. (C) Using siE2F8, E2F8 knockdown was performed in E2F8-elevated HeLa cell lines. (D) The proliferation of HeLa cells transfected with siE2F8 and negative control siNC was determined using the CCK-8 assay. (E) Using shE2F8, E2F8 knockdown was performed in E2F8-elevated ME180 cell lines. (F) The proliferation of ME180 cells transfected with shE2F8 and negative scrambled control was determined using the CCK-8 assay. Each assay was performed in triplicate. Data are means ± standard deviation. ** P <0.01, ***P<0.001 vs siNC and scrambled control.
Human Epithelioid Cervical Adenocarcinoma (Hela) Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human epithelioid cervical adenocarcinoma (hela) cells/product/Korean Cell Line Bank
Average 90 stars, based on 1 article reviews
human epithelioid cervical adenocarcinoma (hela) cells - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
hpv-infected cervical cancer cell line hela  (Korean Cell Line Bank)
90
Korean Cell Line Bank hpv-infected cervical cancer cell line hela
E2F8 expression in cervical cancer cell lines and correlation with suppressed cell proliferation. (A, B) The expression levels of E2F8 RNA and protein were significantly higher in some cervical cancer cell lines (CaSki, <t>HeLa,</t> and ME180) compared to that in normal control cells. (C) Using siE2F8, E2F8 knockdown was performed in E2F8-elevated HeLa cell lines. (D) The proliferation of HeLa cells transfected with siE2F8 and negative control siNC was determined using the CCK-8 assay. (E) Using shE2F8, E2F8 knockdown was performed in E2F8-elevated ME180 cell lines. (F) The proliferation of ME180 cells transfected with shE2F8 and negative scrambled control was determined using the CCK-8 assay. Each assay was performed in triplicate. Data are means ± standard deviation. ** P <0.01, ***P<0.001 vs siNC and scrambled control.
Hpv Infected Cervical Cancer Cell Line Hela, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hpv-infected cervical cancer cell line hela/product/Korean Cell Line Bank
Average 90 stars, based on 1 article reviews
hpv-infected cervical cancer cell line hela - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
human cervical cancer (hela) cell line  (Pasteur Institute)
90
Pasteur Institute human cervical cancer (hela) cell line
E2F8 expression in cervical cancer cell lines and correlation with suppressed cell proliferation. (A, B) The expression levels of E2F8 RNA and protein were significantly higher in some cervical cancer cell lines (CaSki, <t>HeLa,</t> and ME180) compared to that in normal control cells. (C) Using siE2F8, E2F8 knockdown was performed in E2F8-elevated HeLa cell lines. (D) The proliferation of HeLa cells transfected with siE2F8 and negative control siNC was determined using the CCK-8 assay. (E) Using shE2F8, E2F8 knockdown was performed in E2F8-elevated ME180 cell lines. (F) The proliferation of ME180 cells transfected with shE2F8 and negative scrambled control was determined using the CCK-8 assay. Each assay was performed in triplicate. Data are means ± standard deviation. ** P <0.01, ***P<0.001 vs siNC and scrambled control.
Human Cervical Cancer (Hela) Cell Line, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cervical cancer (hela) cell line/product/Pasteur Institute
Average 90 stars, based on 1 article reviews
human cervical cancer (hela) cell line - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
human epithelial cancer cervical cell line tzm-bl  (Ocera Inc)
90
Ocera Inc human epithelial cancer cervical cell line tzm-bl
E2F8 expression in cervical cancer cell lines and correlation with suppressed cell proliferation. (A, B) The expression levels of E2F8 RNA and protein were significantly higher in some cervical cancer cell lines (CaSki, <t>HeLa,</t> and ME180) compared to that in normal control cells. (C) Using siE2F8, E2F8 knockdown was performed in E2F8-elevated HeLa cell lines. (D) The proliferation of HeLa cells transfected with siE2F8 and negative control siNC was determined using the CCK-8 assay. (E) Using shE2F8, E2F8 knockdown was performed in E2F8-elevated ME180 cell lines. (F) The proliferation of ME180 cells transfected with shE2F8 and negative scrambled control was determined using the CCK-8 assay. Each assay was performed in triplicate. Data are means ± standard deviation. ** P <0.01, ***P<0.001 vs siNC and scrambled control.
Human Epithelial Cancer Cervical Cell Line Tzm Bl, supplied by Ocera Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human epithelial cancer cervical cell line tzm-bl/product/Ocera Inc
Average 90 stars, based on 1 article reviews
human epithelial cancer cervical cell line tzm-bl - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
cervical cancer cell line skg-iiia  (BioResource International Inc)
90
BioResource International Inc cervical cancer cell line skg-iiia
E2F8 expression in cervical cancer cell lines and correlation with suppressed cell proliferation. (A, B) The expression levels of E2F8 RNA and protein were significantly higher in some cervical cancer cell lines (CaSki, <t>HeLa,</t> and ME180) compared to that in normal control cells. (C) Using siE2F8, E2F8 knockdown was performed in E2F8-elevated HeLa cell lines. (D) The proliferation of HeLa cells transfected with siE2F8 and negative control siNC was determined using the CCK-8 assay. (E) Using shE2F8, E2F8 knockdown was performed in E2F8-elevated ME180 cell lines. (F) The proliferation of ME180 cells transfected with shE2F8 and negative scrambled control was determined using the CCK-8 assay. Each assay was performed in triplicate. Data are means ± standard deviation. ** P <0.01, ***P<0.001 vs siNC and scrambled control.
Cervical Cancer Cell Line Skg Iiia, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cervical cancer cell line skg-iiia/product/BioResource International Inc
Average 90 stars, based on 1 article reviews
cervical cancer cell line skg-iiia - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
cervical cancer cell lines  (YUTAKA Engineering Corporation)
90
YUTAKA Engineering Corporation cervical cancer cell lines
E2F8 expression in cervical cancer cell lines and correlation with suppressed cell proliferation. (A, B) The expression levels of E2F8 RNA and protein were significantly higher in some cervical cancer cell lines (CaSki, <t>HeLa,</t> and ME180) compared to that in normal control cells. (C) Using siE2F8, E2F8 knockdown was performed in E2F8-elevated HeLa cell lines. (D) The proliferation of HeLa cells transfected with siE2F8 and negative control siNC was determined using the CCK-8 assay. (E) Using shE2F8, E2F8 knockdown was performed in E2F8-elevated ME180 cell lines. (F) The proliferation of ME180 cells transfected with shE2F8 and negative scrambled control was determined using the CCK-8 assay. Each assay was performed in triplicate. Data are means ± standard deviation. ** P <0.01, ***P<0.001 vs siNC and scrambled control.
Cervical Cancer Cell Lines, supplied by YUTAKA Engineering Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cervical cancer cell lines/product/YUTAKA Engineering Corporation
Average 90 stars, based on 1 article reviews
cervical cancer cell lines - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


A. Representative images of HeLa and HEK293T cells showing localization of BG4, the G4 binding antibody to mitochondria following immunofluorescence study. Nucleus is stained with DAPI (blue color), mitochondria with MitoTracker DR (red) and BG4 with Alexa-Fluor 488 (green). A merged image is shown with merge of red and green as depicted by Coste’s mask (colocalization is represented as a white dot). B, C. Quantitation showing colocalization of BG4 with MitoTracker indicated as dot plots. The colocalization was quantified using Mander’s colocalization coefficient (ImageJ software) analyzing a minimum of 100 cells as red over green (B) and green over red (C). D. Representative image of rho(0) cell showing localization of BG4 to mitochondria following immunofluorescence as investigated in panel A. E. Quantitation showing comparison of colocalization of BG4 between HeLa cells and rho(0) cells is shown as dot plots. The colocalization was quantified using Mander’s colocalization coefficient analyzing a minimum of 50 cells as red over green. F. BG4 bound mtDNA was purified after reverse crosslinking and used for real-time PCR using primers derived from different regions of the mitochondrial genome, which include 5 G-quadruplex forming regions and 10 random regions. Input DNA served as template control. No antibody control was also used. Bars in blue (first 5) are for G-quadruplex forming regions, while in green (last 10) are for random regions. Y-axis depicts threshold Ct value obtained following real time PCR for each primer. Error bar represents mean ± SEM. G. Agarose gel profile showing the amplification of Input DNA (left panel) and BG4 pull down DNA (right panel). “M” denotes 100 bp ladder.

Journal: bioRxiv

Article Title: Unleashing a Novel Function of Endonuclease G in Mitochondrial Genome Instability

doi: 10.1101/2021.05.27.445952

Figure Lengend Snippet: A. Representative images of HeLa and HEK293T cells showing localization of BG4, the G4 binding antibody to mitochondria following immunofluorescence study. Nucleus is stained with DAPI (blue color), mitochondria with MitoTracker DR (red) and BG4 with Alexa-Fluor 488 (green). A merged image is shown with merge of red and green as depicted by Coste’s mask (colocalization is represented as a white dot). B, C. Quantitation showing colocalization of BG4 with MitoTracker indicated as dot plots. The colocalization was quantified using Mander’s colocalization coefficient (ImageJ software) analyzing a minimum of 100 cells as red over green (B) and green over red (C). D. Representative image of rho(0) cell showing localization of BG4 to mitochondria following immunofluorescence as investigated in panel A. E. Quantitation showing comparison of colocalization of BG4 between HeLa cells and rho(0) cells is shown as dot plots. The colocalization was quantified using Mander’s colocalization coefficient analyzing a minimum of 50 cells as red over green. F. BG4 bound mtDNA was purified after reverse crosslinking and used for real-time PCR using primers derived from different regions of the mitochondrial genome, which include 5 G-quadruplex forming regions and 10 random regions. Input DNA served as template control. No antibody control was also used. Bars in blue (first 5) are for G-quadruplex forming regions, while in green (last 10) are for random regions. Y-axis depicts threshold Ct value obtained following real time PCR for each primer. Error bar represents mean ± SEM. G. Agarose gel profile showing the amplification of Input DNA (left panel) and BG4 pull down DNA (right panel). “M” denotes 100 bp ladder.

Article Snippet: HeLa (human cervical cancer), MEF (mouse embryonic fibroblast) and HEK293T (human embryonic kidney epithelial cell line) were purchased from National Centre for Cell Science, Pune, India.

Techniques: Binding Assay, Immunofluorescence, Staining, Quantitation Assay, Software, Comparison, Purification, Real-time Polymerase Chain Reaction, Derivative Assay, Control, Agarose Gel Electrophoresis, Amplification

E2F8 expression in cervical cancer cell lines and correlation with suppressed cell proliferation. (A, B) The expression levels of E2F8 RNA and protein were significantly higher in some cervical cancer cell lines (CaSki, HeLa, and ME180) compared to that in normal control cells. (C) Using siE2F8, E2F8 knockdown was performed in E2F8-elevated HeLa cell lines. (D) The proliferation of HeLa cells transfected with siE2F8 and negative control siNC was determined using the CCK-8 assay. (E) Using shE2F8, E2F8 knockdown was performed in E2F8-elevated ME180 cell lines. (F) The proliferation of ME180 cells transfected with shE2F8 and negative scrambled control was determined using the CCK-8 assay. Each assay was performed in triplicate. Data are means ± standard deviation. ** P <0.01, ***P<0.001 vs siNC and scrambled control.

Journal: International Journal of Biological Sciences

Article Title: E2F8 regulates the proliferation and invasion through epithelial-mesenchymal transition in cervical cancer

doi: 10.7150/ijbs.37686

Figure Lengend Snippet: E2F8 expression in cervical cancer cell lines and correlation with suppressed cell proliferation. (A, B) The expression levels of E2F8 RNA and protein were significantly higher in some cervical cancer cell lines (CaSki, HeLa, and ME180) compared to that in normal control cells. (C) Using siE2F8, E2F8 knockdown was performed in E2F8-elevated HeLa cell lines. (D) The proliferation of HeLa cells transfected with siE2F8 and negative control siNC was determined using the CCK-8 assay. (E) Using shE2F8, E2F8 knockdown was performed in E2F8-elevated ME180 cell lines. (F) The proliferation of ME180 cells transfected with shE2F8 and negative scrambled control was determined using the CCK-8 assay. Each assay was performed in triplicate. Data are means ± standard deviation. ** P <0.01, ***P<0.001 vs siNC and scrambled control.

Article Snippet: Human epithelioid cervical adenocarcinoma (HeLa) cells were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing, Control, Knockdown, Transfection, Negative Control, CCK-8 Assay, Standard Deviation

E2F8 promotes cell migration and invasion. (A, C) Wound healing assay observed under the optical microscope was used to determine cell migration using si and shE2F8. E2F8 knockdown was performed in E2F8-high HeLa and ME180 cell lines. (B, D) Cell invasion was observed under the optical microscope. Matrigel invasion assays were used to determine invasion after 48 h in HeLa and ME180 cells. Each assay was performed in triplicate. Data represent means ± standard deviation. ** P <0.01, *** P <0.001 vs. siNC and scrambled control.

Journal: International Journal of Biological Sciences

Article Title: E2F8 regulates the proliferation and invasion through epithelial-mesenchymal transition in cervical cancer

doi: 10.7150/ijbs.37686

Figure Lengend Snippet: E2F8 promotes cell migration and invasion. (A, C) Wound healing assay observed under the optical microscope was used to determine cell migration using si and shE2F8. E2F8 knockdown was performed in E2F8-high HeLa and ME180 cell lines. (B, D) Cell invasion was observed under the optical microscope. Matrigel invasion assays were used to determine invasion after 48 h in HeLa and ME180 cells. Each assay was performed in triplicate. Data represent means ± standard deviation. ** P <0.01, *** P <0.001 vs. siNC and scrambled control.

Article Snippet: Human epithelioid cervical adenocarcinoma (HeLa) cells were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Migration, Wound Healing Assay, Microscopy, Knockdown, Standard Deviation, Control